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Er:YAG ·¹ÀÌÀú Á¶»ç°¡ Streptococcus mutansÀÇ Áõ½Ä¾ïÁ¦¿¡ ¹ÌÄ¡´Â È¿°ú

INHIBITORY EFFECT OF Er:YAG LASER ON THE GROWTH OF STREPTOCOCCUS MUTANS

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Abstract

Er:YAG ·¹ÀÌÀúÀÇ ±¸°­³» »ê »ý¼º ¼¼±ÕÀÎ S. mutans¿¡ ´ëÇÑ »ì±ÕÈ¿°ú¸¦ Æò°¡Çϱâ À§ÇÏ¿© S. mutans KCTC 3065°¡ Æ÷ÇÔµÈ ¼¼±Õ¹è¾ç¾×¿¡ 650§­ Á÷°æÀÇ Á¶»ç beamÀ» °®´Â Er:YAG ·¹ÀÌÀú¸¦ ºñÁ¢ÃË½Ä ¹æ¹ýÀ¸·Î, Á¶»ç¼¼±â(50mJ, 100mJ, 150mJ)¿Í pulse repetition rate(5Hz, 10Hz, 20hz). ±×¸®°í Á¶»ç½Ã°£(1ÃÊ, 3ÃÊ, 5ÃÊ)À» ´Þ¸®ÇÏ¿© Á¶»çÇÏ°í À̶§ÀÇ ¼¼±Õ ¹è¾ç¾×ÀÇ ¿Âµµº¯È­, ¼¼±Õ ±º¶ô¼ö, »ê »ý¼º´É, ºÒ¿ë¼º ¼¼Æ÷¿Ü ´Ù´ç·ùÀÇ ¾çÀ» ÃøÁ¤ÇÑ °á°ú ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù.
10§¡ ¼¼±Õ ¹è¾ç¾×¿¡¼­´Â ·ÊÀÌÀú¸¦ Á¶»çÇÑ ±ºÀÌ ·¹ÀÌÀú¸¦ Á¶»çÇÏÁö ¾ÊÀº ´ëÁ¶±º¿¡ ºñÇØ Àü¹ÝÀûÀ¸·Î ¼¼±ÕÀÇ ±º¶ô¼ö°¡ °¨¼ÒµÇ¾úÀ¸¸ç Á¶»ç¼¼±â¿Í pulse repetition rate°¡ Ŭ¼ö·Ï, ±×¸®°í Á¶»ç½Ã°£ÀÌ ±æ¾îÁú¼ö·Ï ¼¼±Õ ±º¶ô¼ö°¡ ºñ·ÊÇÏ¿© °¨¼ÒÇÏ´Â °æÇâÀ» º¸¿´´Ù. ±×·¯³ª 100§¡ ¼¼±Õ ¹è¾ç¾×¿¡¼­´Â ·¹ÀÌÀú¸¦ Á¶»çÇÏÁö ¾ÊÀº ´ëÁ¶±º¿¡ ºñÇØ ¼¼±Õ ±º¶ô¼öÀÇ º¯È­°¡ Àû¾ú´Ù.
10§¡ ¼¼±Õ ¹è¾ç¾×¿¡¼­´Â ·¹ÀÌÀú Á¶»ç¿¡ ÀÇÇØ S. mutansÀÇ »ê »ý¼º´ÉÀÌ ÀÏÁ¤½Ã°£ µ¿¾È ¾ïÁ¦µÇ¾úÀ¸³ª 100§¡ ¼¼±Õ ¹è¾ç¾×¿¡¼­´Â ·¹ÀÌÀú¸¦ Á¶»çÇÏÁö ¾ÊÀº ´ëÁ¶±º¿¡ ºñÇØ »ê »ý¼º´ÉÀÇ º¯È­°¡ °üÂûµÇÁö ¾Ê¾Ò´Ù. 10§¡ ¼¼±Õ ¹è¾ç¾×¿¡¼­´Â ·¹ÀÌÀú Á¶»ç¿¡ ÀÇÇØ S. mutansÀÇ ºÒ¿ë¼º ¼¼Æ÷¿Ü´Ù´ç·ùÀÇ ÇÕ¼ºÀÌ ºÎºÐÀûÀ¸·Î °¨¼ÒµÇ¾úÀ¸³ª 100§¡¼¼±Õ ¹è¾ç¾×¿¡¼­´Â º¯È­°¡ °üÂûµÇÁö ¾Ê¾Ò´Ù.
ÀÌ¿Í °°Àº »ç½Ç¿¡ ±Ù°ÅÇØ º¼ ¶§ ·¹ÀÌÀú´Â S. mutans¿¡ ´ëÇÑ Áõ½Ä¾ïÁ¦È¿°ú¸¦ °¡Áö°í ÀÖÀ¸¸ç ±× ±âÀüÀº ÃÊÀ½ÆÄ µî¿¡ ÀÇÇÑ ±â°èÀû ÀÛ¿ëÀÌ ¾Æ´Ñ ¿­ÀÛ¿ë¿¡ ÀÇÇÑ °ÍÀÓÀ» ÃßÁ¤ÇØ º¼ ¼ö ÀÖ´Ù.
The purpose of this study is to investigate the sterilization effect of Er:YAG laser against the intraoral acid producing bacterium, S. mutans, by irradiating the culture solution containing S. mutans KCTC 3065 with Er:YAG laser having a 650gm diameter beam through the non-contact method. We obtained the following results after examining the temperature changes of the culture solution, numbers of bacterial colonies, and acid-producing ability and attaching ability on teeth by measuring the amount of extracellular polysaccharide produced by S. mutans.
The number of bacterial colony was decreased in 1041 culture solution irradiated with laser in overall compared to the control solution. The number decreased as the irradiation intensity and pulse repetition rate were larger and as the exposure time was increased. However, it did not change significantly in 100pi culture solution compared to the control solution.
Although the acid-producing ability of S. mutans was inhibited for a certain duration after laser irradiation in 10pl bacterial culture solution, it did not change in 10041 solution compared with the control solution.
The amount of extracellular polysaccharide synthesized by S. mutans was partially decreased through laser irradiation in 10/1 culture solution but did not change in 100;1 culture solution.
Based on these findings, we concluded that Er:YAG laser has an sterilization effect on S. mutans in which we presume that the mechanism is through the heat effect rather than the mechanical effect from development of ultrasound.

Å°¿öµå

Er:YAG ·¹ÀÌÀú;Streptococcus mutans;Er:YAG laser;Streptococcus mutans

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